On Thu, 10 May 2018 13:28:12 -0600, Bill in Co wrote:
There is an option with some defrag utilites to select boot time directory
consolidation, which moves all the directories together at the beginning of
the disk partition, instead of leaving them scattered throughout the disk
(based on when they were created).

I read that doing so is beneficial, especially for FAT32 volumes (presumably
in terms of access times to get to the folders and files).

My current setup is this:
C: system and boot up (the main partition). NTFS (60GB)
D: FAT32 partition for misc files (40 GB)
E: FAT32 partition for music files (40 GB)
F: FAT32 partition for video files (80 GB)

My question is this. I did that on my D: FAT32 partition (C: is the NTFS
system partition). Is all the information on where the directories and
files are stored on D: contained on the D: partition, or is it also stored
in the root C: partition in its MFT?

If you meant software settings which are file paths, then yes. Most would
likely be stored in drive C: or the system partition. Some would be in files
within user profile folders, and some would be in the Windows registry.